Actresses Lexa Doig and Lisa Ryder are both regular cast members on the Gene Roddenberry sci-fi series Andromeda. Their roles in Jason X are reversals of their roles on Andromeda as Lexa Doig is an android on Andromeda and human in Jason X while Lisa Ryder is a human in Andromeda but an android in Jason X.
Director James Isaac makes an uncredited cameo appearance as a doctor in this film.
Screenwriter Todd Farmer makes a small appearance in the film as Dallas, one of the E-X grunts.
Actor/director David Cronenberg makes a guest appearance in the film as Doctor Wimmer. Cronenberg is also known for directing the 1981 "exploding head trauma" film Scanners as well as the 1989 remake of The Fly.